High Throughput Label-Free One-Bead-One-Compound (OBOC) Screening Assay
Produces label-free assay for screening Produces true sense of binding through elimination of false-positive interactions
Label-free screening assay
Traditional OBOC screening assays are currently performed by attaching a fluorescent or enzyme based label on a target to determine its binding to a library of test compounds. OBOC libraries have been used to identify a nearly limitless number of macromolecule-binding compounds, including compounds that are useful against cancers. While current methods of OBOC screening have successfully detected new synthetic compounds or ligands, these methods have several shortcomings. Specifically: (i) the attachment of a label can interfere with binding, (ii) the label may sterically inhibit parts of the target macromolecule and (iii) the process of attaching a label can alter the structure of the target macromolecule. Researchers at the University of California, Davis have developed a high throughput, label-free OBOC screening assay. Compared to current methods, this assay discriminates between beads that bind to the target by staining target molecules directly with either silver or fluorescent stains. After target binding, the beads are then gelled and pulled off the target through electrophoresis. The target molecules migrate a short distance from their bounded bead and appear as a ‘comet’ or streak of staining within the gel. This unique combination of staining and electrophoresis provides previously unknown benefits for OBOC screening.
20160313311
Additional Technologies by these Inventors Tech ID/UC Case 27247/2014-564-0 Related Cases 2014-564-0
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