Methods for Selecting Plants After Genome Editing
Does not require tissue culture Does not require selectable transgenes Selects genome edits on a whole-plant level
Selection for genome edits generated by TALENs, CRISPR-Cas9 systems and zinc-finger nucleases
Technologies for precise genome modification based on zinc finger nucleases, TALENs, and CRISPR-Cas9 systems are advancing rapidly, allowing researchers to efficiently target genetic changes to specific DNA sequences. CRISPR-mediated gene modification efficiency can be as high as 98% with minimal off-target effects and has now been demonstrated in several plants including Arabidopsis, tobacco, sorghum, rice, maize and wheat. However, (with the exception of Arabidopsis) it is currently necessary to go through tissue culture to obtain modifications of the germline; tissue culture is frequently mutagenic and therefore there is the likelihood of introducing unpredictable and off-target mutations in addition to the desired changes. Also, it remains challenging to select for cells with the targeted genome modification. UC Davis researchers have developed a method that does not require tissue culture and allows the selection of modified plants without the use of selectable transgenes in order to be able to introduce alleles into well adapted genotypes without background mutations.
20170029831
Related Materials US Patent Application Publication Tech ID/UC Case 24209/2014-585-0 Related Cases 2014-585-0
美国
