Method for construction of normalized cDNA libraries
Efficient and high yield reduction in prevalent clone frequency Normalizes large sized cDNAs containing considerable protein coding information -- Important for new drug and drug target discovery 3’ non coding sequences are involved in the reassociation reaction eliminating the possibility of losing rare clones to cross hybridization with sequences of more prevalent members of the same gene family
Normalized cDNA library construction for new gene identification Generation of normalized cDNA libraries from various tissues, in various species, and at different stages of development
This technology represents a process for normalizing cDNA libraries from tissues of human or animal origin. It makes use of the preferential hybridization of prevalent cDNAs and the separation of single stranded from duplexed cDNAs to reduce the freq...
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