"DiGEP" Technology for Phosphoprotein Analysis
To resolve these issues, researchers at Purdue University have developed a novel gel-based process, called Differential Gel Electrophoresis of Phosphoproteome (DiGEP). This process uses metal ions, such as Ti4+, Zr4+, Fe3+, and Ga3+, which are attracted to phosphate groups and attach fluorophores on the phosphoproteins. This ultimately helps visualize phosphorylation changes in different samples on the same gel. DiGEP analyses are advantageous because they enable the visualization of phosphoproteins on a single gel and select only relevant proteins for in-gel digestion and mass spectrometric analysis. It is highly specific, selective, and quantitative and can be used routinely in labs for quantitative phosphorylation measurement, in vitro kinase assay, kinase and phosphatase activity assay, kinase/phosphatase inhibitor screening, and detection of in vivo phosphorylation. Not only will this technology help a general biological laboratory to effectively measure changes in protein phosphorylation, but will also help pharmaceutical and biotech industries to develop effective diagnostic and therapeutic agents relating to kinases.
Visualization of phosphoproteinsSelection of proteins for spectrometric analysis Effective and quantitative measures
Research & Development LabsDisease Research
W. Andy Tao - BiochemistryW. Andy Tao - ChemistryW. Andy Tao - Medicinal Chemistry and Molecular PharmacologyTao Research GroupPurdue BiochemistryPurdue ChemistryPurdue Medicinal Chemistry and Molecular Pharmacology
United States
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美国
