Drug Delivery Vehicle for Treatment of Eye Disease: Glaucoma
Human carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and is associated with glaucoma (a major cause of blindness).Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, is attached to nanoceria particles using epichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein) is also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo.X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successful derivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocal fluorescence microscopy.
生物医学
医药成分
美国
