CELL-MATRIX MICROSPHERES, METHODS FOR PREPARATION AND APPLICATIONS
A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modelling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.
Patent Number: US8679809B2
Application Number: US2007750863A
Inventor: Chan, Barbara Pui | Chan, Godfrey Chi-Fung | Wong, Hoi Ling | Cheung, Pik To | Cheah, Song-Eng Kathryn | Chan, Danny
Priority Date: 19 May 2006
Priority Number: US8679809B2
Application Date: 18 May 2007
Publication Date: 25 Mar 2014
IPC Current: C12N001104 | C12N0005077 | C12N00050775
US Class: 435177 | 4240937
Assignee Applicant: The University of Hong Kong
Title: Cell-matrix microspheres, methods for preparation and applications
Usefulness: Cell-matrix microspheres, methods for preparation and applications
Summary: The cell-matrix microsphere can be used for producing a biomolecule and for injecting or implanting into tissues of animals or humans (claimed), for tissue repair or regeneration, e.g. for skin wound or an injured cartilage. It can also be used as therapeutics, and as three-dimensional (3D) microcarriers. The cell-matrix microspheres can also be used to immobilize other therapeutic molecules, e.g. factors inducing specific differentiation lineage such as Transforming Growth Factor (TGF) beta for chondrogenic differentiation, and anti-inflammatory drugs.
Novelty: New cell-matrix microspheres comprises an extracellular matrix forming a nanofibrous microsphere, useful for producing a biomolecule and for injecting or implanting into tissues of animals or humans for tissue repair or regeneration
诊断/治疗
其他疾病
香港
