Label-Free Optical Sensing And Characterization Of Biomolecules By D8 Or D10 Metal Complexes
The Present Invention Provides A Composition For Detecting And/Or Characterizing A Multiple-Charged Biomolecule Comprising A Charged D8 Or D10 Metal Complex, Wherein The Metal Complex Electrostatically Binds To The Multiple-Charged Biomolecule To Induce Aggregation And Self-Assembly Of The Metal Complex Through Metal . . . Metal Interactions, [Pi] . . . [Pi] Interactions, Or A Combination Of Both Interactions. The Present Invention Further Provides Assay Methods And Kits For Label-Free Optical Detection And/Or Characterization Of Biomolecules Carrying Multiple Charges, E.G., Single-Stranded Nucleic Acids, Polyaspartate, Polyglutamate, Using A Composition Comprising A Charged D8 Or D10 Metal Complex.
Patent Number: US8057989B2
Application Number: US2007625109A
Inventor: Yam, Vivian Wing-Wah | Yu, Cong | Chan, Kenneth Hoi-Yiu | Wong, Keith Man-Chung
Priority Date: 10 Feb 2006
Priority Number: US8057989B2
Application Date: 19 Jan 2007
Publication Date: 15 Nov 2011
IPC Current: C12Q000168 | C12Q000100
US Class: 435004 | 4350061 | 435006
Assignee Applicant: The University of Hong Kong
Title: Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes
Usefulness: Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes
Summary: For detecting and characterizing a multiply-charged biomolecule, e.g. single-stranded nucleic acid, protein or polypeptide, in a sample (claimed). Also useful for detecting structural changes of target single-stranded nucleic acid and detecting mutation in a target single-stranded nucleic acid.
Novelty: Composition for detecting charged biomolecule comprises charged metal complex with 8 or 10 electrons in d-orbital, which electrostatically binds to biomolecule to induce aggregation and self-assembly via metal-metal and pi-pi interactions
生物医学
DNA /基因工程
香港
