Method and System for Real Time Quantification and Monitoring of Nucleic Acid Amplification Using Electroconductive or Electrochemically Active Labels利用具有導電能力或電化學活性的標記物構建起對核酸擴增過程進行即時監測、定量分析的方法及其裝置
The present invention describes an electrochemistry-based real-time polymerase chain reaction (PCR). This invention aims to address the deficiencies inherent in the prior art of optical-based (e.g. fluorescence) real-time PCR method by utilizing the novel strategies based on redox-labeled peptide nucleic acid probe and solid phase extension of oligonucletotide capture probe with redox markers to realize the real time electrochemical PCR technique.
This invention also contains a microchip platform strategy to carry out the electrochemistry-based real-time PCR on a portable chip based system. This electrochemical approach utilizes simple and miniaturizable instrumentation compared to the bulky and sophisticated optics required in the fluorescence-based schemes. This invention can be used for the real-time bioanalyte (e.g. water pathogens, E. Coli) detection in a decentralized environment.
1. Simple instrumentation and cost effective
2. Electroactive reporters cost less than the fluorescence-based counterparts
3. Procedure for multiplex DNA analysis in a microarray format is simple
4. Real-time - allows simultaneous amplification and multiplexing detection in short assay time
5. High throughput
6. Accurate - uses threshold cycle detection in order to have accurate quantification
7. Portable - allows decentralizing analysis from central laboratory to on-spot detection
8. Wide dynamic range of quantification
- Market of this new technology will cover scientific research, clinical diagnostics, environmental monitoring, food testing and biodefense applications.
- Two types of electrochemistry-based real time PCR instruments are possible for different applications:-
(a) Bench-top type - for hospitals, research/testing/teaching laboratories, government lab for food/drug testing which require real time PCR facilities.
(b) Portable real-time PCR analyzer - promises nucleic acids biosensor for decentralized and point-of-care testing, including real-time bioanalyte detection such as:
- genetic diseases analysis;
- viral quantification;
- pathogen detection / identification;
- environmental monitoring;
- drug monitoring
Patent Number: US7465926B2
Application Number: US2006537280A
Inventor: Stassinopoulos, Epaminondas G.
Priority Date: 29 Sep 2006
Priority Number: US7465926B2
Application Date: 29 Sep 2006
Publication Date: 16 Dec 2008
IPC Current: G01T000116
US Class: 2503361
Assignee Applicant: The United States of America as represented by the Administrator of the National Aeronautics and Space Administrationshington
Title: Device system and method for miniaturized radiation spectrometer
Usefulness: Device system and method for miniaturized radiation spectrometer
Summary: System for sensing radiation across a broad energy spectrum.
Novelty: Radiation sensing system, has high and low linear energy transfer detectors coupled to CPU via analog-to-digital converters, respectively, where detectors are assembled within single unit
生物医学
DNA /基因工程
11 Feb 2009
US 12/377124
US 8465926
TTC.PA.314S
香港
