Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- 技術優勢
- Detects as few as a single copy of a target polyribonucleotide
- 技術應用
- Detect a target nucleotide (e.g., of a pathogen in a biological sample) Purify a particular target RNA (or RNA protein complex) from within a complex mixture Delivery of modular components (e.g., effector domains) in conjunction with Cas9 Modulate expression of RNA molecules in eukaryotic cells RNA processing enzyme
- 詳細技術說明
- None
- *Abstract
-
DNA restriction enzymes transformed molecular biology in the 1970s by making it possible to cleave specific DNA sequences at will. Sequencing of RNA molecules currently entails copying the RNA into a DNA strand that is then sequenced by conventional methods. This approach, also known as RNASeq, is robust and can yield many millions of sequence reads. However, the necessity of generating cDNA introduces inherent bias due to sequence-dependent efficiencies of individual steps.
UC Berkeley researchers discovered variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids that can be used to detect the presence of a particular sequence in a polyribonucleotide, (e.g., to detect the presence of pathogen in a biological sample). . The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.
- *IP Issue Date
- Aug 25, 2015
- *Principal Investigation
-
Name: Jennifer Doudna
Department:
Name: Rachel Haurwitz Smoligovets
Department:
Name: Blake Wiedenheft
Department:
Name: Martin Jinek
Department:
- 申請號碼
- 9115348
- 其他
-
Related Materials
Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease
Additional Technologies by these Inventors
- Methods and Compositions for Controlling Gene Expression by RNA Processing
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Methods and Compositions for Using Argonaute to Modify a Single-Stranded Target Nucleic Acid
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- Cas9 Variants With Altered DNA Cleaving Activity
- Split-Cas9 For Regulatable Genome Engineering
- Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
- Methods For High Signal-To-Noise Imaging Of Chromosomal Loci In Cells Using Fluorescent Cas9
- Identification Of Sites For Internal Insertions Into Cas9
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- Small Molecule Assisted Cell Penetrating Cas9 RNP Delivery
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Cas12c/C2C3 Compositions and Methods of Use
- A Dual-RNA Guided CasZ Gene Editing Technology
- Endoribonucleases For Rna Detection And Analysis
Tech ID/UC Case
19837/2010-028-0
Related Cases
2010-028-0
- 國家/地區
- 美國
