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Next Generation Pathogen Diagnostic Panel

詳細技術說明

ApplicationsMultiplex diagnostic panel for bovine infectious diseaseDetects the presence of 45 of the most common pathogensA multiplicity of sample types can be used, includingtissues, blood, milk and swabs Problems AddressedMore sensitive and specific than traditional culture-baseddiagnosticsCan screen a sample for multiple pathogens simultaneouslyEasy data analysisFast and Inexpensive Pathogens Validated (additional pathogens being tested)  Respiratory Pathogens Enteric Pathogens Repro Pathogens Mastitis BVDV (with typing), other pestiviruses, IBR (vs vaccine strain), Bovine Coronavirus, Mycoplasma species, Influenza D, PI3, BRSV, Adenovirus 3, Histophilus somni, Pasturella multocida, Mannheimia haemolytica, Trueperella pyogenes,  Bibersteinia trehalosi E. coli toxins (F41, F5, sta, stx 1,2, eae, cnf 1/2, alpha hly) Salmonella, Rotavirus (A,B,C), Johne’s, Clostridium perfringens toxin typing (alpha, beta, beta2, epsilon, iota, cpe), Giardia, Cryptosporidium, Coronavirus Neospora, Leptospirosis species, Toxoplasma, Chlamydia species, Campylobacter fetus fetus and venerealis, BHV-4, IBR, BVDV, Brucella abortus, T. foetus, Bluetongue/EHD, Anaplasma marginale, Listeria monocytogenes, Ureaplasma Staphylococcus aureus, E. coli toxins, Streptococcus agalactiae, Mycoplasma bovis, Prototheca, Streptococcus uberis, Streptococcus dysgalactiae, Coag negative Staphylococcus, Pseudomonas, Klebsiella, Zygomycetes,  Aspergillus, Nocardia

*Abstract

Summary

The advantages of a nucleic acid-based technique, such asPCR, are numerous and include speed, sensitivity and specificity. However, PCRhas its own challenges in that it is limited by pathogens that can be detectedin a single reaction. To address that concern, UGA researchers have developed aNext Generation Sequencing (NGS)-based diagnostic panel that is able to detectthe presence of 45 different pathogens using target-specific primers forPCR-mediated amplification. This multiplex panel has been validated usingclinical samples that were subsequently tested with commonly used diagnostictechniques.  These results confirm thevalidity of using NGS-based techniques in veterinary diagnostics.

國家/地區

美國