Bacterial Hexose Formation: Sugar conversions, one-carbon fixation and the accumulation of sugar-derived products in Escherichia coli
- 詳細技術說明
- ApplicationsGenetically engineered E. coli which converts pentoses into hexoses, via capture ofone-carbon compounds (e.g., CO2, CH3OH, etc.). The microorganismaccumulates that hexose and makes it available to metabolism into products ofinterest, such as commodity chemicals and rare/unnatural sugars. Seeking partnerships leading toengineering these strains to produce fermentation products of interest tocommercial partners ProblemsAddressed (benefits/advantages)Glucose is the primarycurrency of energy for much of life. Plant, bacterial and mammalian cells haveevolved highly efficient biochemical pathways to oxidize glucose, not only togenerate energy but also to synthesize precursor molecules used as the buildingblocks of cellular materials. Many cells have also devised mechanisms to storeglucose in different forms: plants generate starch and cellulose, while manyeukaryotic cells accumulate glycogen. In addition to precursor molecules andstorage products, structurally diverse compounds are derived from glucose andother monosaccharides; for example, glycosylation of small molecules andproteins provides unique cellular functionalities. Promoting hexoseaccumulation beyond natural levels constitutes an opportunity for betterunderstanding of monosaccharide interconversions, and as a platform for thegeneration – in much higher yields - ofimportant products derived from these intermediate hexoses. Theaccumulation of hexoses directly from pentoses might advance the use oflignocellulosic hydrolysates with organisms such as Saccharomyces cerevisiaewhich metabolize D-glucose readily but are natively unable to consume pentoses.
- *Abstract
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TechnologySummary
The group led by UGA’s Mark Eitemandeveloped bacterial strains which accumulate a six carbon sugar("hexose") such as glucose and mannose as a final product in a mediumwhen supplied with five carbon sugars ("pentose") such as xylose orarabinose or even when supplied with glycerol.
Since the cells can berendered unable to metabolize certain sugars but otherwise still grow andfunction biologically, this invention affords the opportunity to direct thesehexoses into valuable products such as the so-called "rare"(unnatural) sugars (e.g., talose, ribose, lyxose, all of relevance in thedevelopment of therapeutics) and to a wide variety of glycosylated (glucuro-,gluco-, galacto-, manno-derivatized) compounds such as cyanidin 3-glucoside. Furthermore, this invention serves as a meansof building onto a carbon backbone from, for example, 5-carbon to 6-carbonlength.
Inventors
Dr. Mark Eiteman
TechnologyDevelopment and IP Status
Technologysuccessfully implemented under both continuous process and batch process
Massconversion yields have been demonstrated to exceed 30% (>0.30 g/g) [see attached, supplemental "Yield Comparison" file. Strains identification has been removed]
Straincan be further modified to overexpress compounds of industrial interest
Providesfor the production of rare, industrially important sugars.
Patent Pending
- 國家/地區
- 美國
