亞洲知識產權資訊網為知識產權業界提供一個一站式網上交易平台，協助業界發掘知識產權貿易商機，並與環球知識產權業界建立聯繫。無論你是知識產權擁有者正在出售您的知識產權，或是製造商需要購買技術以提高操作效能，又或是知識產權配套服務供應商，你將會從本網站發掘到有用的知識產權貿易資訊。

返回搜索結果

Determination of Isoaspartate in a Peptide

總結: Researchers at Purdue University have developed a fast and inexpensive method to determine isoaspartate by chemical derivatization in the backbones of peptides by ionizing labeled peptides. This method is the simplest and most accessible assay for isoAsp determination, which makes it useful to researchers studying its link to disease or drug-bioconjugate degradation.

技術應用: Research related to isoAsp's link to disease or drug-bioconjugate degradationMedical/HealthPharmaceutical industry

*Background: In vitro and in vivo isomerization of aspartate (Asp) to isoaspartate (isoAsp) is one of the main routes to protein degradation. Deamidation of asparagine (Asn) can also yield isoAsp via a common succinimide intermediate. Not only can this transformation change the structure and activity of a protein, it can also lead to immunological response. Furthermore, an increase in endogenous isoAsp has been linked to Alzheimer's disease and aging. The body naturally produces enzymes that work to reduce the levels of isoAsp, namely PIMT and PCMT, both of which methylate isoAsp to encourage isomerization to Asp. The spontaneous, post-translational generation of isoAsp poses an immediate problem to the development of protein-bound pharmaceuticals, where shelf life and activity may be directly affected; in fact, isoAsp generation is one of the most common contributors to heterogeneity in a protein-bound drug. Factors that induce the generation of isoAsp include pH, secondary and tertiary protein structure, and formulation.

*Stage of Development: Concept validated through theory, simulation, or models

*Web Links: Purdue Office of Technology CommercializationPurdue Innovation and EntrepreneurshipR. Graham CooksPurdue ChemistryAston Labs

欲了解更多信息，請點擊這裡