Cas12c/C2C3 Compositions and Methods of Use
- 技術優勢
- Adds additional versatility Variant PAM
- 技術應用
- Genome editing Genetic engineering Gene therapy Research tools (e.g., high-throughput screening of gene functions in cell lines and in vivo) Creation of transgenic animal models
- 詳細技術說明
- None
- *Abstract
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The CRISPR-Cas system is now understood to confer bacteria and archaea with acquired immunity against phage and viruses. CRISPR-Cas systems consist of Cas proteins, which are involved in acquisition, targeting and cleavage of foreign DNA or RNA, and a CRISPR array, which includes direct repeats flanking short spacer sequences that guide Cas proteins to their targets. Class 2 CRISPR-Cas systems are streamlined versions in which a single Cas protein bound to RNA is responsible for binding to and cleavage of a targeted sequence. The programmable nature of these minimal systems has facilitated their use as a versatile technology that is revolutionizing the field of genome manipulation, so there is a need in the art for additional Class 2 CRISPR/Cas systems (e.g., Cas protein plus guide RNA combinations).
Researchers have shown that a Cas12c protein (also referred to as a Cas12c polypeptide or a C2c3 polypeptide) complex as well as Cas12c variants can be used for specific binding and cleavage of DNA. The Cas12c complex utilizes a novel RNA and a guide RNA to perform double stranded cleavage of DNA. Similar to CRISPR Cas9, Cas12c enzymes are expected to have a wide variety of applications in genome editing and nucleic acid manipulation.
- *Principal Investigation
-
Name: Jillian Banfield
Department:
Name: David Burstein
Department:
Name: Janice Sha Chen
Department:
Name: Jennifer Doudna
Department:
Name: Lucas Benjamin Harrington
Department:
Name: David Paez-Espino
Department:
- 其他
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Additional Technologies by these Inventors
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Methods and Compositions for Controlling Gene Expression by RNA Processing
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Methods and Compositions for Using Argonaute to Modify a Single-Stranded Target Nucleic Acid
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- Cas9 Variants With Altered DNA Cleaving Activity
- Split-Cas9 For Regulatable Genome Engineering
- Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
- Methods For High Signal-To-Noise Imaging Of Chromosomal Loci In Cells Using Fluorescent Cas9
- Identification Of Sites For Internal Insertions Into Cas9
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- Small Molecule Assisted Cell Penetrating Cas9 RNP Delivery
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- A Dual-RNA Guided CasZ Gene Editing Technology
- Endoribonucleases For Rna Detection And Analysis
Tech ID/UC Case
28893/2018-040-0
Related Cases
2018-040-0
- 國家/地區
- 美國
