A Peel-able Array for Affinity-based Separation of Biomaterials
- 詳細技術說明
- A method of using an array of parylene-based "peel-able" strips to screen and capture aptamers (or other biomaterial) from a mixture onto specific proteins immobilized on the strips, and subsequently isolate and recover each separated aptamer of interest by simply peeling off each strip
- *Abstract
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Microarrays are affinity-based assays that allow for massively parallel screening of candidate biomolecules in a library. However, it remains a challenge to reliably isolate and recover multiple biomolecular species. Cornell researchers have come up with a method of using an array of parylene-based "peel-able" strips (Peel-Strips) to screen and capture aptamers (or other biomaterial) from a mixture onto specific proteins immobilized on the Peel-Strips, and subsequently isolate and recover each separated aptamer of interest by simply peeling off each strip. The novel technique is based on a polymer layer that is functionalized to enable the immobilization of any protein/DNA. By integration with microfluidic structures, a microarray format was created that allows for parallel screening/capture of multiple biomolecular interactions and subsequent separation and recovery of the species of interest.
Potential Applications
- Screening and recovery of protein-protein, protein-cells interactions, and nucleic acids hybridization and even stem cells, pluripotent stem cells
- Drug screening
- Platform for cataloging and storage
Advantages
- Easy-to-use
- No specialized equipment (optics, electronics) to recover the samples are required
- Does not mechanically destroy sample such as due to scraping the surface as used in existing methods
- *Licensing
- Martin Teschlmt439@cornell.edu(607) 254-4454
- 其他
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Patent applications: WO2011130511; 13/641,206
- 國家/地區
- 美國
