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Novel Method for Complete Heme Incorporation into Recombinant Proteins

詳細技術說明

Cornell researchers have developed a novel method that provides complete heme incorporation in recombinant proteins, greatly improving their functionality.

*Abstract

Cornell researchers have developed a novel method that provides complete heme incorporation in recombinant proteins, greatly improving their functionality.

This invention overcomes the common problem of incomplete heme incorporation into recombinant proteins by co-expression of ferrochelatase in E. coli with the addition of delta-ALA in the growth medium. By co-expressing ferrochelatase, the enzyme that inserts iron into heme, the yield and purity of full-functional heme proteins increases considerably; the heme is in fact 100% incorporated. The addition of delta-ALA is also critical because its production would normally be the rate-limiting step for heme biosynthesis.

 

Potential Applications:

• Therapeutic protein manufacturing.
• Research applications.

*Licensing

PhillipOwh607-254-4508po62@cornell.edu

其他

• U.S.Patent Patent No. 9,085,766

• J.Sudhamsu, M. Kabir, M. V. Airola, B. A. Patel, S. Yeh, D. L. Rousseau, B. R.Crane. Co-expression of ferrochelatase allows for complete heme incorporationinto recombinant proteins produced in E.coli. ProteinExpr Purif 73(1):78-82. doi:10.1016/j.pep.2010.03.010.

國家/地區

美國