A Cell-Based Method for Rapidly Monitoring Protein Folding in Bacterial Cells Based on the Tat Translocator
- 詳細技術說明
- The invention relates to compositions and methods for analyzing and altering protein folding and solubility.
- *Abstract
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The invention describes a genetic method for monitoring protein folding directly in bacterial cells. Using recombinant DNA technology, a "target" protein of interest can be cloned into a reporter plasmid and introduced into bacteria. The novelty of the invention is that the "target" protein is specifically targeted to the twin-arginine translocation (tat) pathway of Escherichi coli using a highly specific signal sequence. Since the tat pathway has the inherent ability to selectively transport only those proteins which have folded properly (e.g., have a high degree of stable secondary structure), the act of transport can be used as a readout for the folding status of the "target" protein. Whereas all existing cellular screens for protein folding reply on indirect measure of protein folding, this is the first genetic screen of its type which assays protein folding directly.
- *Licensing
- PhillipOwh607-254-4508po62@cornell.edu
- 其他
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US patent US8,722,584
Patent Application US20080287315
- 國家/地區
- 美國
