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Continuous, enhanced detection of droplet contents in electrical impedance spectroscopy

技術優勢

Continuous amplified detection in separate droplets Does not require fluorescent dye

技術應用

Label-free detection of DNA Nanoparticle-enhanced detection of droplet contents

詳細技術說明

Amplified detection of DNA in droplets has been demonstrated using a fluorescent probe. The use of fluorescent markers is expensive and requires extra processing steps. In addition, fluorescence intensity results must be compared to a calibration curve. Electrical impedance spectroscopy (EIS) has been used for the detection of flowing whole cells or static subcellular components. The inventors at UCI have developed a method and system that allows the detection of a gene in genomic DNA and provides a platform that can detect DNA in flowing droplets. The technique incorporates molecular affinity forces to align molecules within droplets at the interface, resulting in increased EIS sensitivity. The method also allows for continuous measurements in traveling microfluidic droplets. The process is ideal for entities using polymerase chain reaction (PCR), as the technique is label-free and provides the capability to detect PCR amplification in many samples in real time.

*Abstract

The inventors at UCI have developed a method and system to make enhanced electrical impedance spectroscopy measurements in a continuously flowing train of microfluidic droplets. The technique increases the sensitivity of the electrical impedance spectroscopy measurements, lowering detection limits and increasing the frequency of continuous measurements.

*IP Issue Date

Nov 19, 2015

*Principal Investigation

Name: Abraham Lee

Department:

Name: Melinda Simon

Department:

申請號碼

20150330927

其他

State Of Development

The system for both continuous microfluidic droplet generation and electrical impedance spectroscopy measurements has been developed and tested.

Tech ID/UC Case

27049/2014-743-0

Related Cases

2014-743-0

國家/地區

美國