Synthetic transcriptional control elements
- 技術優勢
- Easy to designOperate on entire operonsHighly amenable to development and modificationOffer a broader dynamic range for expression of the regulated nucleic acid of interest than with cis-regulators of translation aloneAllow for precise regulation of the level of expression of the nucleic acid of interest
- 技術應用
- Gene expression control systems for bioengineeringCustomizable regulators for gene expression
- 詳細技術說明
- None
- *Abstract
-
Much of bacterium’s regulatory potential is included in 5’-untranslated regions (5’-UTRs), which control the expression of physically adjacent downstream genes. When engineering custom regulators for 5’-UTRs, there is a tradeoff between ease of design for regulators of translation and versatility of output for regulators of transcriptional continuation. Scientists at UC Berkeley have developed a method for converting cis-regulators of translation into synthetic translation-coupled regulators of transcription and related compositions. The compositions and methods involve a nucleic acid construct including a synthetic translation-coupled regulator of transcription (synthetic TCRT) that includes a cis-regulator of translation coupled to an adapter. The resulting custom regulators are easy to design, can operate on entire operons and can be composed into logics and higher-order functions.
- *IP Issue Date
- Mar 14, 2017
- *Principal Investigation
-
Name: Adam Arkin
Department:
Name: Chang Liu
Department:
Name: Lei Qi
Department:
- 附加資料
- Inventor: QI, Lei S. | LIU, Chang | ARKIN, Adam P.
Priority Number: WO2013049330A1
IPC Current: C07H002102 | C12N001500
Assignee Applicant: The Regents of the University of California
Title: SYNTHETIC TRANSCRIPTIONAL CONTROL ELEMENTS AND METHODS OF GENERATING AND USING SUCH ELEMENTS | ÉLÉMENTS SYNTHÉTIQUES DE CONTRÔLE TRANSCRIPTIONNEL ET MÉTHODES DE GÉNÉRATION ET D'UTILISATION DE CES ÉLÉMENTS
Usefulness: SYNTHETIC TRANSCRIPTIONAL CONTROL ELEMENTS AND METHODS OF GENERATING AND USING SUCH ELEMENTS | ÉLÉMENTS SYNTHÉTIQUES DE CONTRÔLE TRANSCRIPTIONNEL ET MÉTHODES DE GÉNÉRATION ET D'UTILISATION DE CES ÉLÉMENTS
Summary: The nucleic acid construct, cell, method or kit is useful for converting cis-regulator of translation into synthetic TCRT. The composition and method are also useful for synthetic gene regulation. The synthetic TCRT is used to regulate the expression of a downstream nucleic acid sequence of interest.
Novelty: New nucleic acid construct useful for converting cis-regulator of translation into synthetic translation-coupled regulator of transcription, comprises synthetic translation-coupled regulator of transcription
- 主要類別
- 生物醫學
- 細分類別
- DNA /基因工程
- 申請號碼
- 9593338
- 其他
-
Publication
Additional Technologies by these Inventors
Tech ID/UC Case
21379/2011-117-0
Related Cases
2011-117-0
- 國家/地區
- 美國
