T Cell Trafficking Genes Identification Using the Sleeping Beauty Transposon
- 詳細技術說明
- Researchershave applied the Sleeping Beauty (SB)transposon system to identify novel candidate genes involved in T-celltrafficking. The SB transposon can identify the genetic drivers that couldincrease the trafficking of T-cells out of the blood and into the tumor.Advantages for using the SB transposon are that it results in mutagenesis ofthe entire genome, but only in T-cells. This can be followed by easy identification of insertion sites in genesthat may be associated with infiltration of T-cells into the tumor, and so maycontribute to the anti-tumor T-cell response. Studies to date demonstrate thatT-cell specific SB mutagenesis can be used to identify candidate genes that maycontribute to the anti-tumor T-cell response. A number of these candidate genes are currently being validated. Genesidentified by the SB transposon have the potential to serve as therapeutictargets to enhance the efficacy of checkpoint blockade therapy for patientswith a broad range of cancers. This technology will have a therapeutic anddiagnostic application in the fields of immunology, oncology and cancerresearch.
- *Abstract
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BackgroundInformation
Immunotherapyis a type of cancer treatment designed to boost the patient’s immune system tofight off cancer cells. An example of immunotherapy is the use of immunecheckpoint blockade antibodies, which enhance T-cell anti-tumor activity andhave produced durable results in treatment of a number of cancers includinglate-stage malignant melanoma. However,therapeutic success is only achieved in about 30% of patients, andunderstanding why has become a focal point in immunotherapy research. Studiesshow that intratumoral T-cell infiltration in tumors improves the efficacy ofcheckpoint blockade therapies. Researchers at The University of Iowa havedeveloped a novel genetic screen approach to identify genes impacting T-celltrafficking in tumors, which can be used as therapeutic targets to enhancecheckpoint blockade therapy.
TechnologySummary
Researchershave applied the Sleeping Beauty (SB)transposon system to identify novel candidate genes involved in T-celltrafficking. The SB transposon can identify the genetic drivers that couldincrease the trafficking of T-cells out of the blood and into the tumor.Advantages for using the SB transposon are that it results in mutagenesis ofthe entire genome, but only in T-cells. This can be followed by easy identification of insertion sites in genesthat may be associated with infiltration of T-cells into the tumor, and so maycontribute to the anti-tumor T-cell response. Studies to date demonstrate thatT-cell specific SB mutagenesis can be used to identify candidate genes that maycontribute to the anti-tumor T-cell response. A number of these candidate genes are currently being validated. Genesidentified by the SB transposon have the potential to serve as therapeutictargets to enhance the efficacy of checkpoint blockade therapy for patientswith a broad range of cancers. This technology will have a therapeutic anddiagnostic application in the fields of immunology, oncology and cancerresearch.
Advantages
· SleepingBeauty Transposon causes both gain-of-function and loss-of-function mutations.
· Tissue-specifictransposase expression
· Therapeuticand diagnostic applications in the fields of immunology, oncology and cancerresearch.
· SBtransposon can identify genes that have the potential to augment intratumoralT-cell infiltration.
Related technology:UIRF Case 2012-069
- *Licensing
- Shannon Sheehan PhD, MBAUIRF Managershannon-sheehan@uiowa.edu319-335-4605
- 國家/地區
- 美國
