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Reagents for typing nucleic acids

總結: Lead Inventors: Philip Goelet, Ph.D.; Michael R. Knapp, Ph.D.Problem or Unmet Need:Current techniques in genetic analysis predominately use enzymes or nucleic acids to recognize DNA fragments produced from sequence-specific cleavage by restriction enzymes. Isolating nucleic acids, particularly those which are related to a biological process or condition of interest, can be difficult due to a lack of suitable hybridization probes and information concerning the structure or identity of the target nucleic acid. This technology presents methods and reagents for developing a library of probes whose hybridization patterns to targeted nucleic acids may be used for genetic analysis. The hybridization probe library consists of mixtures of oligonucleotides with random and pseudorandom sequences. The method can be used with restriction digestion to generate a restriction map of a nucleic acid without sequencing.

技術優勢: No sequencing required Generates library of hybridization probes with greater ease

技術應用: Genetic analysis (i.e. fingerprinting) o Forensic medicine o Paternity identification o Lineage analysis o Allelotyping o Biopsy analysis o Diagnosis of disorders and infectious diseases Restriction mapping

詳細技術說明: This technology presents methods and reagents for developing a library of probes whose hybridization patterns to targeted nucleic acids may be used for genetic analysis. The hybridization probe library consists of mixtures of oligonucleotides ...

*Inquiry: Cynthia Lang Columbia Technology Ventures Tel: (212) 854-8444 Email: TechTransfer@columbia.edu

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