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In vitro and in vivo Transduction of Intact Pre-implantation Mammalian Embryos

詳細技術說明: This invention simplifies genetic engineering in mammalian research and commercial settings by introducing nucleic acids into mammalian embryos without the need for conventional mechanical methods, mi

*Abstract: This invention simplifies genetic engineering in mammalian research and commercial settings by introducing nucleic acids into mammalian embryos without the need for conventional mechanical methods, microinjection or electroporation, which can be lethal. The new transgenic platform works simply by exposing AAV viral particles to an "intact" pre-implantation embryo. Using this method minimizes the need for labor-intensive embryo harvesting and transferring into pseudopregnant females.

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