Fosill and ShARC: Two New Methods for Making Long-Distance (~40kb) Jumping Libraries for High-Quality Next Generation Genome Sequencing
- 詳細技術說明
- None
- *Abstract
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When next generation sequencing methods are applied to large genomes, they are not yet as accurate as the older, more expensive methods. To figure out the long-range connections between the many short DNA fragments that next generation sequencers read, scientists rely on ΓÇÿjumping libraries.ΓÇÖ But efforts to make libraries with long enough pieces (10-40kb) to produce accurate, high-quality genome assemblies have been stymied by technical hurdles. Now scientists at the Broad Institute have developed new techniques for producing jumping libraries that overcome these hurdles. The invention consists of two new methods, ShARC and Fosill, for making jumping libraries with sufficient long (~40kb) DNA inserts to produce high-quality human genome assemblies using next generation sequencers. The techniques increase the yield of unique 40kb strands with readable end pairs 2 to 5 orders of magnitude over traditional in vitro techniques with the same amount of starting material. In addition, the ends of these 40kb strands can be sequenced using high-speed, low-cost next-generation instruments.
- 國家/地區
- 美國
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