A Dual-RNA Guided CasZ Gene Editing Technology
- 技术优势
- Adds additional versatility because of small size Variant PAM
- 技术应用
- Genome editing Genetic engineering Gene therapy Research tools (e.g., high-throughput screening of gene functions in cell lines and in vivo) Creation of transgenic animal models
- 详细技术说明
- None
- *Abstract
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The CRISPR-Cas system is now understood to confer bacteria and archaea with acquired immunity against phage and viruses. CRISPR-Cas systems consist of Cas proteins, which are involved in acquisition, targeting and cleavage of foreign DNA or RNA, and a CRISPR array, which includes direct repeats flanking short spacer sequences that guide Cas proteins to their targets. Class 2 CRISPR-Cas systems are streamlined versions in which a single Cas protein bound to RNA is responsible for binding to and cleavage of a targeted sequence. The programmable nature of these minimal systems has facilitated their use as a versatile technology that is revolutionizing the field of genome manipulation, so there is a need in the art for additional Class 2 CRISPR/Cas systems (e.g., Cas protein plus guide RNA combinations).
UC Berkeley researchers discovered a new type of Cas protein, CasZ. (CasZ) is short compared to previously identified CRISPR-Cas endonucleases, and thus use of this protein as an alternative provides the advantage that the nucleotide sequence encoding the protein is relatively short. The researchers have shown that the CRISPR CasZ protein and its variants can be used in a complex for specific binding and cleavage of DNA. The CRISPR CasZ complex utilizes a novel RNA and a guide RNA to perform double stranded cleavage of DNA and the complex is expected to have a wide variety of applications in genome editing and nucleic acid manipulation.
- *Principal Investigation
-
Name: Jillian Banfield
Department:
Name: David Burstein
Department:
Name: Janice Sha Chen
Department:
Name: Jennifer Doudna
Department:
Name: Lucas Benjamin Harrington
Department:
Name: David Paez-Espino
Department:
- 其他
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Additional Technologies by these Inventors
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Methods and Compositions for Controlling Gene Expression by RNA Processing
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Methods and Compositions for Using Argonaute to Modify a Single-Stranded Target Nucleic Acid
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- Cas9 Variants With Altered DNA Cleaving Activity
- Split-Cas9 For Regulatable Genome Engineering
- Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
- Methods For High Signal-To-Noise Imaging Of Chromosomal Loci In Cells Using Fluorescent Cas9
- Identification Of Sites For Internal Insertions Into Cas9
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- Small Molecule Assisted Cell Penetrating Cas9 RNP Delivery
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Cas12c/C2C3 Compositions and Methods of Use
- Endoribonucleases For Rna Detection And Analysis
Tech ID/UC Case
28913/2018-045-0
Related Cases
2018-045-0
- 国家/地区
- 美国
