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Nucleic Acid Sequence Detection and Capture by Formation of an Abasic Site-Derived Cross-Link

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*Abstract

University of MissouriOffice of Technology Management & Industry RelationsNon-Confidential Abstract of InventionUM Disclosure No. 17UMC064Nucleic Acid Sequence Detection and SNP Capture by Formation of an Abasic Site-Derived Crosslink INNOVATION:MU researchers have invented an improved method to capture single-stranded DNA or RNA using a sequence-specific cross-linking reaction to covalently capture target nucleic acids with single base pair sensitivity. This technology enables a unique approach to Single Nucleotide Polymorphism (SNP) detection, and has broad potential in nanopore and/or PCR based nucleic acid detection. BACKGROUND:Specific detection of single nucleotide variations/polymorphisms in nucleic acid sequences is important within the realm of research and diagnostics. Standard probe hybridization approaches rely on the cumulative attraction of the probe to the target conferred by hydrogen bonding between their respective individual nucleotides. With standard probe lengths of >15 base pairs needed to create sufficient binding specificity it is difficult to design capture probes capable of distinguishing between two target nucleic acids that differ by only a single nucleotide. The technology described herein provides a unique and improved method to capture SNP-specific sequences using the additional discriminatory power of a SNP-specific abasic site derived crosslink.APPLICATIONS:• Single nucleotide polymorphism detection/capture• Single nucleotide variation detection/capture• Nucleic Acid crosslinkingADVANTAGES:• Durable covalent crosslinking• Use of nanopore technology• Applications in PCR enrichment• Improved SNP sensitivityPATENT STATUS:• Patent PendingLICENSING POTENTIAL:• University seeks licensee with the potential to commercializeINVENTOR(S):• Kent Gates• Li-qun Gu• Maryam Imani Nejad• Xinyue Zhang• Ruicheng ShiPUBLICATIONS• Price NE, Johnson KM, Wang J, Fekry MI, Wang Y, Gates KS. Interstrand DNA−DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA. JACS 136:3483-3490, 2014.• Second publication in submissionCONTACT INFO: Office of Technology Management & Industry Relations Brian Buntaine, MS, MBA Senior Licensing & Business Development Associate Email: buntaineb@missouri.edu Phone: 573-882-0470

*Principal Investigation

Name: Kent Gates, Professor

Department:

Name: Li-qun (Andrew) Gu, Ast Professor

Department:

Name: Maryam Imani Nejad, Graduate Student

Department:

Name: Xinyue Zhang, Postdoc Fellow

Department:

Name: Ruicheng Shi, Graduate Student

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其他

国家/地区

美国