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Quantitative Peptide Microarray Technology

技术应用

By printing different sets of peptides on a microarray, this technology can be used for defining protein binding specificity, illustrating protein-protein interactions, determining epitopes recognized by antibodies, and identifying substrates of various enzymes.

详细技术说明

Scientists at UC San Diego have developed a microarray technology providing a quantitative, high-throughput, and cost-effective way to conduct proteomics measurements. The present invention employs a microarray technology that prints peptides on a glass surface thereby significantly reducing the size of the array. It requires using fewer peptides than traditional methods, whereby thousands of peptides can be easily printed on one DNA microarray-sized slide, compared to the existing technology’s limit of only tens of peptides on the same surface area. This approach requires a small quantity of protein for analysis, which is particularly useful in disease diagnosis, where sample size is limited. Additionally, quantitative protein-peptide interactions (binding affinities) may be measured from fluorescence probe intensities.

*Abstract

Proteomics is considered the next step in the study of biological systems. It is complicated because the entire complement of proteins in a cell (proteome) differs from cell to cell and from time to time. While peptide arrays have been around for years, they have not been widely applicable to the proteomic studies due to their current limitations. Namely, present array dimensions limit scaling up for proteomic measurements and array output is often qualitative rather than quantitative. Furthermore, the high cost of peptide synthesis combined with limited access to instruments has inhibited the widespread adoption of array technology for proteomics.

*IP Issue Date

May 20, 2014

*Principal Investigation

Name: Wei Wang

Department:

Name: Zheng Xu

Department:

附加资料

Patent Number: US8728980B2
Application Number: US13666284A
Inventor: Wang, Wei | Xu, Zheng
Priority Date: 4 Nov 2011
Priority Number: US8728980B2
Application Date: 1 Nov 2012
Publication Date: 20 May 2014
IPC Current: C40B003004 | G01N003368
US Class: 506009 | 4350071
Assignee Applicant: The Regents of the University of California
Title: Peptide microarray and method of use
Usefulness: Peptide microarray and method of use
Summary: The peptide microarray is useful for epitope mapping; for drug screening or vaccine development; and, in combination with computational predictions, for identifying modified peptides, where the modified peptides are methylated or acetylated peptides corresponding to post-translational modification (PTM) proteins that regulate lysine methylation. The microarray and method are useful for identifying one or more binding peptides interacting with a protein of interest or its domain (all claimed). The vaccine may be used for autoimmune diseases and allergies.
Novelty: Peptide microarray useful for epitope mapping, for drug screening or vaccine development, and for identifying modified peptides, comprises a solid support surface and predicted unique binding peptides

主要类别

化工/材料

细分类别

化工/材料应用

申请号码

8728980

其他

Related Materials

Xu Z, Hou T, Li N, Xu Y, Wang W. 2011. Proteome-Wide Detection of Abl1 SH3 Binding Peptides by Integrating Computational Prediction and Peptide Microarray. Mol Cell Proteomics. 2011 Oct 24. [Epub ahead of print]

Additional Technologies by these Inventors

• A Systems Biology Approach for Identifying Drug Targets

Tech ID/UC Case

22220/2010-063-0

Related Cases

2010-063-0

国家/地区

美国