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Monoclonal Antibody Against Pnpase (Clone 2A2)

技术优势

Specific antibody to detect PNPase (clone 2A2 by antibody-antigen complex formation.

技术应用

Immunocytochemistry/immunofluorescence Immunoprecipitation Western blot

详细技术说明

RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonuclease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. The described mouse monoclonal antibody binds to human mtPAP and has been described for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.

*Abstract

Mouse monoclonal antibody against the human mitochondrial polyribonucleotide nucleotidyltransferase 1 (PNPase). This antibody has been tested for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.

*Principal Investigation

Name: Xuning Guo

Department:

Name: Wen Hwa Lee

Department:

其他

Related Materials

Role of SUV3 helicase in maintaining mitochondrial homeostasis in human cells. J Biol Chem. 2008 Oct 3;283(40):27064-73
Human mitochondrial SUV3 and polynucleotide phosphorylase form a 330-kDa heteropentamer to cooperatively degrade double-stranded RNA with a 3'-to-5' directionality. J Biol Chem. 2009 Jul 31;284(31):20812-21

Additional Technologies by these Inventors

• Monoclonal Antibody Against PNPase (Clone 3H5)
• Monoclonal Antibody Against mtPAP (Clone 3D2)
• Monoclonal Antibodies Against Spc24/25 (Clone 2C8)
• Monoclonal Antibodies Against Spc24/25 (Clone 2A10)
• Monoclonal Antibody Against PNPase (Clone 4C11)

Tech ID/UC Case

25543/2011-376-0

Related Cases

2011-376-0

国家/地区

美国