Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- 技术应用
- Genetic engineering or editing of Cas9 polypeptides Controlling of site-specific gene regulation
- 详细技术说明
- None
- *Abstract
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The ability to program Cas9 for DNA cleavage at sites defined by guide RNAs has led to its adoption as a robust and versatile platform for genome engineering. Whereas there are a number of ongoing successes with using the CRISPR-Cas9 system for genome engineering, there is a need for understanding the structural basis for guide RNA recognition and DNA targeting by Cas9.
UC Berkeley researchers have developed software and methods for providing the structures of Cas9 with and without the polynucleotides bound thereto, and have developed the crystals comprising the Cas9 polypeptides. Using the atomic coordinates, the software can be used to computationally identify a site for amino acid residue substitution, insertion, or deletion to alter a function or chemical property of a Cas9 polypeptide.
- *Principal Investigation
-
Name: Fuguo Jiang
Department:
Name: Samuel Sternberg
Department:
Name: David Taylor
Department:
Name: Jennifer Doudna
Department:
Name: Martin Jinek
Department:
Name: Emine Kaya
Department:
- 其他
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Publication
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Additional Technologies by these Inventors
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Methods and Compositions for Controlling Gene Expression by RNA Processing
- Methods and Compositions for Using Argonaute to Modify a Single-Stranded Target Nucleic Acid
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- Cas9 Variants With Altered DNA Cleaving Activity
- Split-Cas9 For Regulatable Genome Engineering
- Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
- Methods For High Signal-To-Noise Imaging Of Chromosomal Loci In Cells Using Fluorescent Cas9
- Identification Of Sites For Internal Insertions Into Cas9
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- Small Molecule Assisted Cell Penetrating Cas9 RNP Delivery
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Cas12c/C2C3 Compositions and Methods of Use
- A Dual-RNA Guided CasZ Gene Editing Technology
- Endoribonucleases For Rna Detection And Analysis
Tech ID/UC Case
23719/2014-078-0
Related Cases
2014-078-0
- 国家/地区
- 美国
