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Dynamic Microfluidic Assays

技术优势: High separation timescale and resolution: allows for real-time modulation and readout of biomolecule reaction processesDynamic analysis of chemical and structural nature of switching processesShort assay times compared to state-of-the-art alternativesSmall sample required for detectable resultsCompatible with range of molecule separation methods and read-out detectors

技术应用: Design, characterization and engineering of biological probesMicrofluidic western blotAnalytical and diagnostic assaysDynamic assessment of reaction kinetics, interactions, dissociations of given interactionsEngineering of biomaterials, for example biomimetic smart materials

*Abstract: Many biomolecules can exist in more than one stable state or configuration and can switch reversibly between them. Molecular switching may occur spontaneously or in response to an external stimulus, such as change in pH, light, or temperature. Molecular switches have numerous applications in life and engineering sciences, including selective drug administration or molecular electronics, and understanding their switching properties is essential for the development of these applications.

Scientists at UC Berkeley have designed a microfluidic device that dynamically measures molecular switching in real-time, using microfluidic molecular separation techniques. For example, the device has been used to better understand the photophysical properties of the widely used biological probe, green fluorescent protein (GFP). In addition to assaying biomolecule switching, this device can be used to separate and identify proteins in a biological sample, and thus be used as a microfluidic western blot that requires only small sample volumes.

*Principal Investigation: Name: Amy Herr
Department:

Name: Alex Hughes
Department:

Name: Augusto Tentori
Department:

附加资料: Inventor: FAKHRU'L-RAZI AHMADUN | MOHD HALIM SHAH ISMAIL | THOMAS CHOONG SHEAN YAW
Priority Number: MY140158A
IPC Current: C11B000102
Assignee Applicant: Universiti Putra Malaysia
Title: AN IMPROVED CONTINUOUS STERILIZATION PROCESS FOR PALM OIL MILL PROCESSING
Usefulness: AN IMPROVED CONTINUOUS STERILIZATION PROCESS FOR PALM OIL MILL PROCESSING

其他

Publication

Additional Technologies by these Inventors

Tech ID/UC Case

22826/2013-035-0

Related Cases

2013-035-0

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