Cell Surface Engineering/chemoselective Ligation Reaction
- 技术优势
- The reaction can be used to engineer the composition of cell surfaces, as well as cells' interior. Ligations can be performed in aqueous solution under physiological conditions. Unique properties of the reactive partners (abiotic and chemically orthogonal) provide for extreme selectivity of the reaction
- 技术应用
- Chemoselective ligation reaction can be used as a powerful tool for: Gaining insights into biological processes: Probing intracellular interactions. Delivery of detectable labels, and receptor ligands for binding by a target receptor. Provides ways of engineering receptors for viral mediated gene transfer. Synthesis of peptides and other polymers. Exploring therapeutic strategies: Engineered compounds that can provide for pharmacological activity, or can serve as a target for delivery of other molecules. Provides new ways of labeling cells as targets for cancer therapy. Designing synthetic model surfaces and unique materials for biomedical applications: Provides new ways of adhering cells to nonbiological materials, e.g., medical implants.
- 详细技术说明
- None
- *Abstract
-
Also see: E. Saxon and C. R. Bertozzi, Cell Surface Engineering By A Modified Staudinger Reaction, Science 2000 Mar. 17: 287 (5460): 2007-2010.
Selective chemical reactions enacted within a cellular environment can be powerful tools for elucidating biological processes or engineering novel interactions. A chemical transformation that permits the selective formation of covalent adducts among richly functionalized biopolymers within a cellular context is presented. A ligation model after the Staudinger reaction forms an amide bond by coupling of an azide and a specifically engineered triarylphosphine. Both reactive partners are abiotic and chemically orthogonal to native cellular components. Azides installed within the cell surface glycoconjugates by metabolism of a synthetic azidosugar were reacted with a biotinylated triarylphosphine to produce stable cell-surface adducts. The tremendous selectivity of the transformation should permit its execution within a cell's interior, offering new possibilities for probing intracellular interaction.
Scientists at UC Berkeley have developed a novel chemoselective ligation reaction that functions in aqueous solution in addition to organic solvents. The reaction is useful in bioconjugation, polymer and material chemistry, and is a powerful tool for gaining insights into biological processes, exploring therapeutic strategies, designing synthetic model surfaces and novel materials for biomedical application. The new reaction offers several unique advantages, including:
- *IP Issue Date
- May 27, 2003
- *Principal Investigation
-
Name: Carolyn Bertozzi
Department:
Name: Eliana Saxon Armstrong
Department:
- 附加资料
- Patent Number: US6570040B2
Application Number: US2001810864A
Inventor: Saxon, Eliana | Bertozzi, Carolyn
Priority Date: 16 Mar 2000
Priority Number: US6570040B2
Application Date: 16 Mar 2001
Publication Date: 27 May 2003
IPC Current: C07F000950 | C07F00096506 | C07F0009655 | C07F00096561 | C07K000102 | C07K000104 | C07K0001107 | C12N000502
US Class: 568017 | 435136 | 435325 | 502162 | 560233 | 568007 | 568008
Assignee Applicant: The Regents of the University of California
Title: Chemoselective ligation
Usefulness: Chemoselective ligation
Summary: For construction of chemical libraries including peptide libraries); modification of surfaces (such as cell surfaces); modification of molecules under physiological conditions (e.g. in a cellular environment); in synthesis of peptides and other polymers; production of modified amino acids and to selectively attach a small molecule to an engineered or semi-synthetic polymer.
Novelty: Chemoselective ligation for construction of peptide library involves reacting an engineered phosphine having an electrophilic trap with azide to form covalent bond between them by intramolecular rearrangement of aza-ylide intermediate
- 主要类别
- 化工/材料
- 细分类别
- 化工/材料应用
- 申请号码
- 6570040
- 其他
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Tech ID/UC Case
16934/2000-060-0
Related Cases
2000-060-0
- 国家/地区
- 美国
