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Fabrication of A Novel Fiber-optic Single-cell pH Sensor

标题

Capillary Wall Coupled Whispering Gallery Mode Microresonator for Sensing and Lasing Applications

详细技术说明

None

*Abstract

pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 µm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2’, 7’-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12 – 7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field.

*IP Issue Date

Nov 3, 2015

*Principal Investigation

Name: Yinfa Ma

Department:

Name: Honglan Shi, Asst Prof, Research

Department:

Name: Qingbo Yang, Graduate Student

Department:

Name: Hai Xiao, Assoc Professor

Department:

申请日期

Apr 22, 2013

申请号码

9,176,281

其他

国家/地区

美国