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F152C25W mouse hybridoma cell line secreting monoclonal autoantibody binding histones

详细技术说明

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*Abstract

Mouse Anti-Histone Auto-Antibody (clone F152C25W) Histones are the chief organizing proteins of DNA in the nucleus of all eukaryotic cells. They can be grouped into five major classes, whereof four form a nucleosome core particle that DNA is wrapped around, and the fifth organizes the linker DNA between the nucleosomes. Histones are among the most highly conserved proteins between species, which underscores their essential function for life of eukaryotic cells.The current invention developed by researchers at University of Missouri is a mouse monoclonal antibody from an autoimmune mouse model that detects all five classes of histones. The antibody reacts with several vertebrate species, including human, bovine, mouse, hamster, and marsupials, and has been shown to work well with Western blots, indirect immunofluorescence, and ELISA. POTENTIAL AREAS OF APPLICATIONS- Detection of histones by Western and ELISA- Staining of cells using immunofluorescenceMAIN ADVANTAGES OF INVENTION- Hybridoma cell line available- Easy to produce in quantities- Detects all classes of histones- Can be used with multiple vertebrate species- Can be used with a variety of applications- IgG2a k isotype allows for easy affinity purificationSTATE OF DEVELOPMENTReady to useLICENSING POTENTIALUniversity seeks licensee with potential to commercializePATENT STATUSNo patent application on fileTECHNOLOGY INNOVATORKim S. WiseTECHNOLOGY MANAGER CONTACTHarriet F. Francis, MS, JD; francish@missouri.edu; 573-884-0374

*Principal Investigation

Name: Kim Wise, Professor Emeritus, Dept of Molecular Microbiology and Immunology, Univ of MO

Department:

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国家/地区

美国