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Methods for Selecting Plants After Genome Editing

技术优势

Does not require tissue culture Does not require selectable transgenes Selects genome edits on a whole-plant level

技术应用

Selection for genome edits generated by TALENs, CRISPR-Cas9 systems and zinc-finger nucleases

详细技术说明

Technologies for precise genome modification based on zinc finger nucleases, TALENs, and CRISPR-Cas9 systems are advancing rapidly, allowing researchers to efficiently target genetic changes to specific DNA sequences. CRISPR-mediated gene modification efficiency can be as high as 98% with minimal off-target effects and has now been demonstrated in several plants including Arabidopsis, tobacco, sorghum, rice, maize and wheat. However, (with the exception of Arabidopsis) it is currently necessary to go through tissue culture to obtain modifications of the germline; tissue culture is frequently mutagenic and therefore there is the likelihood of introducing unpredictable and off-target mutations in addition to the desired changes. Also, it remains challenging to select for cells with the targeted genome modification. UC Davis researchers have developed a method that does not require tissue culture and allows the selection of modified plants without the use of selectable transgenes in order to be able to introduce alleles into well adapted genotypes without background mutations.

*Abstract

Researchers at UC Davis have discovered methods of selecting plants with targeted genome edits without resorting to tissue culture or selectable transgenes. The resulting method is a non-transgenic approach that avoids the off-target background mutations generated by selection in tissue culture.

*IP Issue Date

Feb 2, 2017

*Principal Investigation

Name: Richard Michelmore

Department:

申请号码

20170029831

其他

Related Materials

US Patent Application Publication

Tech ID/UC Case

24209/2014-585-0

Related Cases

2014-585-0

国家/地区

美国