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Ex vivo activation and expansion of human T cells using biocompatible substrates with tunable rigidity

总结

Autologous T cells are currently being investigated for use in adoptive immunotherapy to treat cancer and infectious diseases. Existing methods to culture, expand, and activate T cells utilize ligands immobilized on stiff surfaces. However, the mechanical properties of culture surfaces influence cell adhesion, proliferation, and differentiation, and may have eventual downstream effects on T cell activity. This technology describes a culture system for the ex vivo expansion of human T cells that utilizes a biocompatible substrate with tunable rigidity. With further development, this system may mimic the cells' native microenvironment, leading to robust T cell activation ex vivo.

技术优势

Biocompatible substrate mimics in vivo microenvironmentEnhances T cell proliferation compared to conventional stiff substratesProduces more cells with a central memory-like phenotypeCan be used for both research and clinical applicationsPatent Information:Patent Pending (WO/2013/036585)Tech Ventures Reference: IR 2941

技术应用

T cell activation for research applicationsT cell activation ex vivo for autologous implantationCancer immunotherapyAutoimmune disease therapy Treatment of chronic infections

详细技术说明

None

*Abstract

None

*Inquiry

Richard NguyenColumbia Technology VenturesTel: (212) 854-8444Email: TechTransfer@columbia.edu

*IR

2941

*Principal Investigation

*Publications

Shen K, Milone MC, Dustin ML, Kam LC. "Nanoengineering of immune cell function" Mater Res Soc Symp Proc. 2009 Jan 1;1209.O'Connor RS, Hao X, Shen K, Bashour K, Akimova T, Hancock WW, Kam LC, Milone MC. "Substrate rigidity regulates human T cell activation and proliferation" J Immunol. 2012 Aug 1;189(3):1330-9.

国家/地区

美国