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Esterase Resistant to Inactivation by Heavy Metals

详细技术说明

The Red Sea brine pools are one of the extreme environments that allow the survival of diverse extremophiles. The Atlantis II brine pool is one of these extreme environments that possesses multiple ha

*Abstract

The Red Sea brine pools are one of the extreme environments that allow the survival of diverse extremophiles. The Atlantis II brine pool is one of these extreme environments that possesses multiple harsh conditions such as; high temperature, salinity, pH and high concentration of multiple, including toxic heavy metals. Therefore the survival of microbial life in such environment utilizing their resistant enzymes makes them an excellent source for tolerant enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool and identified a novel esterase.We report the isolation and biochemical characterization of this unique esterase; EstATII from the lower convective layer of the Atlantis II brine pool. EstATII is a halotolerant, thermophilic and resistant to a spectrum of heavy metals including toxic concentration of metals. To the best of our knowledge, this is the first study to identify and characterize a novel enzyme from the Atlantis II brine pool in the Red Sea.

*Inquiry

Contact Ingy Darwish, licensing officer of the technology transfer office at tto@aucegypt.edu

*Publications

Mohamed YM, Ghazy MA, Sayed A, Ouf A, El-Dorry H, Siam R (2013): Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool. Scientific Reports. 2013 Nov 28;3:3358. doi: 10.1038/srep03358.

国家/地区

美国