3-D Transfected Cell Arrays for High Throughput Transcriptional Profiling
- 技术优势
- High throughputQuantitativeRapid dataacquisition
- 详细技术说明
- Novel method for investigating gene regulation via transcriptional profiling#healthcare #diagnostictool #therapeutics #drugdiscovery #researchtool #method
- *Abstract
-
Northwestern scientists have developed a high throughput method for investigating gene transcription under various conditions. To achieve this, they designed a 3D hydrogel platform within which cells can be seeded and observed over time. This innovative system allows for high throughput screening of multiple transcription factors at once in a rapid and quantitative manner. In laboratory settings, this 3D cell array can be generated using a variety of cell types, which opens up the possibility that patient samples could be studied in-depth via this method. For example, samples from cancer patients could be investigated for drug sensitivity or for accurate disease tracking and diagnosis. This invention represents a step forward for tissue engineering research, but could also be adapted for use as a sensor for environmental toxins or biological warfare agents.
- *Inventors
- Angela K. PannierAbigail D. BellisMichael S. WeissEric A. AriaziV. Craig JordanJulie WielandLonnie Shea*
- *Publications
- 1. Pannier AK, Wieland JA,Shea LD (2008) Surface polyethylene glycol enhances substrate-mediated genedelivery by nonspecifically immobilized complexes, Acta Biomater, 4: 26-39.2. Pannier AK, Ariazi EA,Bellis AD, Bengali Z, Jordan VC, Shea LD (2007) Bioluminescence imaging forassessment and normalization in transfected cell arrays, Biotechnol Bioeng, 98: 486-97.3. Bellis AD,Penalver-Barnabe B, Weiss MS, Yarrington ME, Barbolina MV, Pannier AK, JerussJS, Broadbelt LJ, Shea LD (2011) Cellular Arrays for Large-Scale Analysis ofTranscriptional Factor Activity, BiotechnolBioeng, 108: 395-403.
- 国家/地区
- 美国
