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Technology for Large-Scale DNA Methylation Profiling​

详细技术说明

Our scientists have developed a biomarker discovery platform that allows for genome-wide interrogation of methylation patterns in small DNA samples. The technology is a restriction enzyme-based assay that uses adaptor-specific PCR to amplify the DNA fraction of interest and compare methylation profiles of different samples using microarray technology.

*Abstract

*Publications

DNA methylation profiles in monozygotic and dizygotic twins.  Kaminsky ZA, Tang T, Wang SC, Ptak C, Oh GH, Wong AH, Feldcamp LA, Virtanen C, Halfvarson J, Tysk C, McRae AF, Visscher PM, Montgomery GW, Gottesman II, Martin NG, Petronis A.  Nature Genetics. 2009 Feb;41(2):240-5. doi: 10.1038/ng.286. Epub 2009 Jan 18.Epigenomic profiling reveals DNA-methylation changes associated with major psychosis.  Mill J, Tang T, Kaminsky Z, Khare T, Yazdanpanah S, Bouchard L, Jia P, Assadzadeh A, Flanagan J, Schumacher A, Wang SC, Petronis A.  Am J Hum Genet. 2008 Mar;82(3):696-711. doi: 10.1016/j.ajhg.2008.01.008.Microarray-based DNA methylation profiling: technology and applications.  Schumacher A, Kapranov P, Kaminsky Z, Flanagan J, Assadzadeh A, Yau P, Virtanen C, Winegarden N, Cheng J, Gingeras T, Petronis A.  Nucleic Acids Res. 2006 Jan 20;34(2):528-42. Print 2006.

国家/地区

美国