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Rapid, Cost-Effective Test for Mycobacteria

详细技术说明: Mycobacterial infections can be detected in biological samples (bodily fluids or tissue sample) with standard detection methods such as high-performance liquid chromatography (HPLC), immunoassay, and colorimetric assay. These methods can be used to directly, as well as indirectly, to detect the presence of selenium (HPLC) or selenoprotein (immunoassay or colorimetric assay).

*Abstract: UCF researchers have discovered a rapid, cost-effective method to test samples for mycobacterial infection. This measurement can be obtained by assaying a biological sample from the subject, providing a more cost effective, less labor intensive testing process. Once a mycobacterium is identified, this method can be further used to monitor the infection after treatment with antibiotic.BackgroundBacterial infections are a major global healthcare problem. Mycobacterial infections are believed to be involved in the pathogenesis of many diseases, including inflammatory bowel disease (e.g., CrohnΓÇÖs disease and ulcerative colitis), tuberculosis, Type 1 diabetes mellitus, and multiple sclerosis. A key challenge in obtaining more rapid diagnoses and providing subsequent treatment is that currently available detection methods of mycobacteria require several months of culture incubation and/or molecular techniques, such as polymerase chain reaction (PCR), requiring extensive time, labor, and costs, as well as delays any potential therapeutic intervention.

*Principal Investigation: Name: Saleh A. Naser, Ph.D. , Associate Director, Graduate Affairs & Professor
Department: Burnett School of Biomedical Sciences

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