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Method and Evaluation for Minimization of Mechanical Effects of Impact Velocity for the Optimization of Freezing Quality of Metal Mirror Impact Freezers

技术优势

The device/method:ΓÇóEnables quantification of potential mechanical deformationsΓÇóSignificantly minimizes pre-cooling effects as observed with conventional techniquesΓÇóAccurately measures the distance travelled by a sample before contacting the mirror surfaceΓÇóSignificantly improves the quality and reproducibility of the sample freezing process as compared to prior-art proceduresΓÇóEffectively modulates the approach and the contact velocity of the sample to match profiles of minimal sample distortionΓÇóSecurely controls the approach of the sample to the cooled metal mirror as compared to conventional techniquesΓÇóWould be commercially useful for a wide range of EM applications such as observations of biological and inorganic specimens and their corresponding quality control/failure analysis

详细技术说明

None

*Abstract

The sample deformation during the metal mirror impact freezing cryopreservation technique is common in prior art. This technique is mainly used in the preparation of biological samples of electron microscopic observations. Such a deformation gives rise to inaccurate interpretation of sample characteristics. Therefore, in order to avoid/minimize these deformations, many procedures/techniques have been used in the past. However, these techniques are associated with many limitations such as the formation of ice crystals, in the case of mechanical hard stops, inaccurate monitoring and potentially high distortions, in the case of offset distance adjustment devices, and potential bounce effects in the case of other commonly used techniques. The proposed invention discloses the development and use of a novel device/method for effectively minimizing such mechanical distortion effects arising due to high impact velocity.

*Principal Investigation

Name: Nector Ritzakis

Department:

Name: Jeff Ruberti

Department:

国家/地区

美国