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Full Length Complimentary DNA (cDNA) Clone for Human Hepatocyte Inducible Nitric Oxide Synthase

详细技术说明

None

*Abstract

This invention provides a cDNA clone for human hepatocyte inducible nitric oxide synthase and a process for preparing the same. This process includes inducing nitric oxide synthase in human hepatocytes, identifying human hepatocyte nitric oxide synthase messenger RNA, isolating the human hepatocyte nitric oxide synthase messenger RNA, collecting the human hepatocyte nitric oxide synthase messenger RNA, separating human hepatocyte poly A messenger RNA from the human hepatocyte nitric oxide synthase messenger RNA, constructing a cDNA library for human hepatocyte nitric oxide synthase, screening this cDNA library for human hepatocyte inducible nitric oxide synthase cDNA clones, and converting the human hepatocyte inducible nitric oxide synthase cDNA clones to a plasmid vector for obtaining a substantially full length cDNA clone encoding human hepatocyte inducible nitric oxide synthase. This process further includes sequencing this cDNA, expressing the human hepatocyte inducible nitric oxide synthase cDNA protein in an expression system, and purifying the human hepatocyte inducible nitric oxide synthase cDNA protein. Non-exclusive license available

*Principal Investigation

Name: Timothy Billiar, Chairman

Department: Med-Surgery

Name: David Geller, M.D.

Department: Med-Surgery

Name: Andreas Nussler, M.D.

Department: Med-Surgery

Name: Richard Simmons, M.D.

Department: Med-Surgery

国家/地区

美国