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B-08 Novel Method to Produce Peptide Drug in Plant Seeds

技术优势

ΓùÅ A method to produce genetically stable and maker-gene free transgenic-rice plants ΓùÅ High quality inoculable protein can be providedΓùÅ Safe and cost effective

技术应用

ΓùÅ Health FoodsΓùÅ Pharmaceutical Drug for Autoimmune DiseasesΓùÅ Orally Ingested Drugs

详细技术说明

This is a method to produce genetically stable and maker-gene free transgenic-rice plants by using co-transformation and PCR-based genetic analysis of progeny lines. By combining the genetic analysis with an expression analysis at protein level, researchers have extended the usefulness of this methodology to obtain stable and homozygous transgenic lines within short periods. Including the development of CII peptide-transgenic rice lines with both genotypic and phenotypic stabilities and the evaluation of transgenic rice seeds on accumulation efficiency and immune-modulation potential of the peptide-fusion protein. Transgenic rice seeds steadily accumulated CII and oral administration of the seeds revealed a tendency of suppressing and delaying the IgG2a response against type II collagen in DBA/1 mice.

*Abstract

Researchers at Nagoya University (NU) have found a novel method to produce transgenic plant seeds with a highly expressed and accumulated tolerogenic peptide. Type II collagen (CII) in joint cartilage is known to be a major auto-antigen in human rheumatoid arthritis. Several animal model- and clinical- studies on tolerance-based immunotherapy for arthritis have been conducted by administering synthetic immunodominant peptides through an oral route.

附加资料

Inventor: MATSUDA, Tsukasa | AOKI, Naohito | HASHIZUME, Fujio | HINO, Shingo | KAKEHASHI, Misako
Priority Number: WO2006095749A1
IPC Current: C12N001569 | A01H000100 | A01H000500
Assignee Applicant: National University Corporation Nagoya University
Title: METHOD FOR EXPRESSION AND ACCUMULATION OF PEPTIDE IN PLANT | PROCEDE DESTINE A L'EXPRESSION ET L'ACCUMULATION DE PEPTIDES DANS LES PLANTES
Usefulness: METHOD FOR EXPRESSION AND ACCUMULATION OF PEPTIDE IN PLANT | PROCEDE DESTINE A L'EXPRESSION ET L'ACCUMULATION DE PEPTIDES DANS LES PLANTES
Summary: The vector is useful for obtaining a recombinant plant such as rice, preferably the rice breed "Koshihikari rice", for expression and aggregation of the target peptide in the seed of a plant and for breeding a multicopy strain (claimed).
Novelty: Novel vector comprising gene encoding glutelin family protein and two copies or more of gene encoding target peptide ligated downstream to glutelin family-encoding gene, useful for obtaining recombinant multicopy variety rice plant

主要类别

农业

细分类别

植物

国家/地区

美国