Kit for detection of gene targeted by microRNA, and method for detection of gene targeted by microRNA
- Detailed Technology Description
- None
- Countries
- United States
- Application No.
- US13/704604
- *Abstract
-
Disclosedare: a detection kit for detecting a gene targeted bymiRNA; and a method for detecting a gene targeted by miRNA in a simple mannerwithout the need of carrying out any operation for introducing a gene into acell using a vector. Specifically disclosed is a kit for detecting a genetargeted by microRNA, which comprises a cellextraction reagent, a microRNA-labelingreagent or labeled microRNA, anda reaction reagent with the microRNA-labeling substance. A cell extractis prepared under mild conditions, labeledmiRNA is added to the cell extract, anda labeled substance is collected. In thismanner, mRNA for a genetargeted by miRNA can be collected readily. cDNAis produced from the collected mRNA, and a gene targeted by miRNA is detected using the cDNA.
- *IP Issue Date
- None
- *IP Type
- Utility
- Country/Region
- USA
