Chemiluminescence-Assisted Cell Endocytosis Kinetics Assay
- 技術優勢
- Real-time evaluation Statistical quantification Fast and convenient process
- 技術應用
- Characterize and quantification of intracellular kinetics Drug delivery vector screening
- 詳細技術說明
- UCLA researchers have developed a novel method to monitor the cell endocytosis kinetics using the bioluminescent reaction of firefly luciferase nanocapsules. A kinetic model is established via correlating the bioluminescence intensity to nanoparticle levels and is used to provide real-time quantification of cell internalization process. The bioluminescent platform provides a fast and convenient way to monitor internalization kinetics, also contains potential for drug delivery vector screening.
- *Abstract
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UCLA researchers in the Department of Chemical and Biomolecular Engineering have developed a novel method to monitor the intracellular kinetics using the bioluminescent reaction of nanoparticles.
- *Principal Investigation
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Name: Yunfeng Lu
Department:
Name: Di Wu
Department:
- 其他
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Background
There has been a great interest to deliver molecules (e.g. proteins and genes) into cells, aiming to alter or restore certain function of the cells for applications such as therapeutics, gene editing, and cell reprograming. The majority of studies focus on extracellular concentration and final output, while leave the intracellular events as a black box, leading to treatment failure, poor efficacy and adverse effect. Therefore, quantitative description on the kinetics of intracellular delivery is essential to fundamentally understand biological process, strive rational design and optimal properties for drug development.
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Tech ID/UC Case
29317/2018-361-0
Related Cases
2018-361-0
- 國家/地區
- 美國
