Surface Treatment for Blood-Contacting Materials
- 詳細技術說明
- ApplicationNew technologydeveloped by UGA researchers combines the clot-preventing capabilities ofliquid infused porous materials with the antibacterial effects of Nitrous Oxide.This combination can have great impact in many blood-contacting devices,serving as the base material (for applications such as tubing, catheters,stents, vascular grafts, extracorporeal circulation, urinary catheters, etc.)or coatings of other implantable devices. Liquid infused porous surfaces showhuge potential due to their ability to greatly reduce bacterial adhesion andclotting in blood-contacting devices. Another method to improve thebiocompatibility of these blood-contacting materials is the deployment ofNitric Oxide (NO). NO is a small, naturally produced molecule which the bodyuses to decrease the activation of platelets as they flow in the blood streamand to kill unwanted bacteria. NO is also released from the sinuses as apreventative measure to reduce the risk of infection. Combining the use ofliquid infused materials with the natural antibacterial molecule, NO, creates asynergistic effect that produces the benefits of both methods, while negatingthe protein clotting effects of NO has individually. The infused liquid willprovide a thin barrier between the material and contacting fluids (such asblood), while actively reducing platelet adhesion and activation. The NO in theliquid will also kill any bacteria that have adhered to the surface.
- *Abstract
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TechnologySummary
Thistechnology combines an NO releasing molecule with liquid-infused materials toprovide an ultra-low fouling surface while providing active means of reducingplatelet activation and adhesion and increasing bactericidal activity. The NO molecule,“SNAP,” was incorporated into medical grade silicone rubber tubing through asolvent swelling process. The impregnated tubing was then treated with SiliconeOil (Si – Oil), to provide a liquid-infused final product. Incorporation of theSi – Oil resulted in a number of synergistic effects, such as decreasedleaching of the NO, as well as limiting the immediate burst effect typicallyseen from NO releasing materials. The presence of the Si – Oil, combined with SNAP,drastically reduced fouling of the material. The ability of SNAP-Si oil todecrease adhesion of platelets was proven through extensive testing. While NOalone showed 74% reduction, and Si - Oil alone showed 56% reduction, thecombination of NO with Si- Oil showed an 82% reduction when compared to untreatedtubing. The SNAP-Si oil combination alsoresulted in a 99.3% reduction in bacterial growth on the polymer surface over a7-day study. Overall, this approach allows for the synergistic effect of activeand passive non-fouling approaches to increase the biocompatibility and reduceinfection in existing medical polymers.
TechnologyDevelopment Status
Severalsamples of tubing have been coated with SNAP Si – oil and subjected to testing.The samples have been tested for in vitroplatelet, bacterial and protein adhesion. The tests showed an 82% decrease inplatelet adhesion and a 99.3 ± 0.15 % decrease in bacterial adhesion, ascompared to control silicone rubber tubing. The protein adhesion levels for thesilicone rubber tubing did not increase with the addition of SNAP Si – oil, arecurring problem for other NO releasing materials. Particularly, the SNAP Si –oil did not absorb high levels of fibrinogen.
The invitro adhesion tests were conducted using freshly drawn porcine blood. Acytocompatability test was also performed, using mouse fibroblast cells. Thistest showed no cytotoxic effects from SNAP, Si – oil or the combinationthereof.
Inventors
HiteshHanda, Ph.D.
- Dr.Hitesh Handa is Assistant Professor in the College of Engineering at theUniversity of Georgia. He received hisB.S. in Polymer Science and Chemical Technology from Delhi College ofEngineering, India and his Ph.D. in Material Science and Engineering from WayneState University. He currently leads the Handa Research Group at UGA, where hisarea of focus is translational research for development of medical devicecoatings.
Marcus Goudie
- M.S.Chemical Engineering - University of Pennsylvania
- B.S.Chemical Engineering - University of Michigan
JitendraPant
- M.Eng.Biotechnology, BITS-Pilani, India
- B.Tech.Biotechnology, ICFAI, Dehradun, India
- 國家/地區
- 美國
