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Small RNA Extraction Kit with High Yield

技術應用: Efficient and robust extraction of small RNAs for diagnostic and research purposes.

詳細技術說明: None

*Abstract: Prof. Wenwan Zhong and her lab at UCR have developed a method to recover small RNAs using TiO₂ fibers which results in a 200-fold improvement in yield when compared to commercially available SiO₂ columns.

Fig. 1 Schematic of the steps involved in recovering small RNAs using TiO₂ fibers and a unique protocol to wash and elute the small RNA.

Fig. 2 Extracting miRNA from MDA-MB-231 cells with TiO₂ fibers and SiO₂ PureLink miRNA isolation columns. Higher recoveries of endogenous hsa-miR-21 were found with TiO₂ fibers when compared with PureLink columns.

*Principal Investigation

Name: Luis Jimenez
Department:

Name: Wenwan Zhong
Department:

Name: Yongzan Zheng
Department:

其他

Background

Small RNAs are noncoding RNAs shorter than 100 nucleotides. One of the most commonly studied small RNA families are microRNAs (miRNA) that can bind to target mRNAs and inhibit their translation or induce degradation. A functional study of miRNAs and exploration of their utility as disease markers require miRNA extraction from biological samples, which contain large amounts of interfering compounds for downstream RNA identification and quantification. The most common extraction methods employ silica columns or the TRIzol reagent but these result in low levels of small RNA recovery.

Related Materials
Jimenez, L. et al. Extraction of microRNAs from biological matrices with titanium dioxide nanofibers. Anal Bioanal Chem, October 13, 2017 .

Tech ID/UC Case
29078/2018-130-0

Related Cases
2018-130-0

國家/地區

美國

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