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Downstream Chromatography Purification for AAV
詳細技術說明
To purify all divergent AAV variants, buoyant density gradients such as CsCl, or iso-osmotic medium iodixanol discontinuous gradients are routinely used. Although quite useful in a laboratory setting, these procedures are neither scalable nor easily adapted for Good Manufacturing Practice (GMP) protocols. GMP production Facility at Nationwide Children's Hospital have developed an efficient and reproducible protocol that could be applied to particular AAV serotypes and also allows vectors to be purified with high recovery rates.
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