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Cell Media and Methods for the Cryopreservation and Recovery of Hepatocytes

詳細技術說明

Description: Fresh hepatocytes are used for invitro liver toxicity testing since they possess intact hepatic functions.However, the sources for fresh liver tissue are very limited and freshhepatocytes in suspension have limited life span with greater than 80% loss ofviability after 2h of incubation at 37°C. Researchers at Kansas StateUniversity have developed cell media and a two-step process for thecryopreservation of hepatocytes to extend the life span and allow forpreservation of hepatic functions, i.e. CYP activity and morphology.  This allows researchers to have functionalcells on demand. Additionally, recovery of cryopreserved hepatocytes is often met with aloss of viability and functionality of cells.  Researchers at K-State have also developed a recoverymedia and method to recover viable and fully functional hepatocytes forexperimental use. Utilizing this kit, cells are recovered at a rate of >80%viability.Advantages:No rapid freezing:The developed mediaprevents rapid freezing and ice crystal formation to prevent cell death.Improved cell viability:Recovered cells treated with this kit retainviability better than previous methods.Retains activity: Hepatocytes treatedwith this media kit retain activity during recovery.Use with multiple cell types: Thiskit works well with dog hepatocytes and has been further modified to be usedwith other organs, such as kidney, etc and for other species including humans.Publications:"Mechanistictoxicity assessment of hexahydroisohumulone in canine hepatocytes, renalproximal tubules, bone marrow-derived mesenchymal stem cells, andenterocytes-like cells” Int J Vet Health Sci Res. 4(2): 88-103. Choi K, Koci J,Ortega MT, Jeffery B, Riviere JE and Monteiro-Riviere NA. (2016)“Oxidative stressresponse in canine in vitro liver, kidney and intestinal models with sevenpotential dietary ingredients” Toxicol. Lett. 241:49-59. Choi K, Ortega MT,Jeffery B, Riviere JE and Monteiro-Riviere NA. (2016)Interested parties should contact:Kansas State University Institute for Commercialization (KSU-IC)2005 Research Park Circle Manhattan, KS 66502Tel: 785-532-3900 Fax: 785-532-3909E-Mail: ic@k-state.edu

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國家/地區

美國