Engineering Heat-stable Disease Resistance in Plants
- 詳細技術說明
- This invention describes introduction of specific mutations of the resistance gene, SNC1, in Arabidopsis thaliana and gene N in tobacco that confers heat-stable disease resistance in plants.
- *Abstract
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Disease resistance in plants can often be suppressed by high temperatures. This heat masking has been ascribed in several economically relevant plants. Developing heat-stable, disease resistant plants is advantageous, especially in this time of global climate change, where plants may now be exposed to temperatures that they are unaccustomed to. Specific mutations of the resistance gene, SNC1, in Arabidopsis thaliana and gene N in tobacco confer heat-stable disease resistance. Introducing these variants into plants will make them disease resistant against pathogens at high and low temperatures. While current work has examined these species because this classification of disease resistance genes exist in a number of plant species, including tomato, tobacco, barley, maize, and rice, modifications in other species will likely confer similar changes when mutated.
Potential Applications:
Many of the disease resistance (R) genes in plants contain nucleotide binding (NB) and leucine-rich repeat (LRR) domains. Resistance to specific pathogens is thought to be mediated by these NB-LRR R genes recognizing and interacting with avirulence (Avr) genes in the pathogen. NB-LRR type R genes can be identified for each pathogen and then mutated and screened for heat-stable disease resistance. Mutations can be introduced into the R gene by site-directed mutagenesis and these R variants can then be introduced to crops to increase their disease resistance across temperatures.
Advantages:
- This is the only available method to create heat-stable disease resistance in plants.
- This technology outlines the mechanism behind the temperature sensitivity of defense responses.
- *Licensing
- JeffFearn607-254-4502jcf55@cornell.edu
- 其他
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- Patents issued inthe U.S. under the number 9,238,680and in China under the number ZL200980157212.8
- Zhu, Y.,Qian, W., & Hua, J. (2010). Temperature modulates plant defense responsesthrough NB-LRR proteins. PLoSPathogens. 6:e1000844.
- 國家/地區
- 美國
