Multiplexed Micro-columns for Improved Aptamer Selection
- 詳細技術說明
- A new approach was developed for identifying aptamers for multiple target molecules simultaneously, offering a major improvement over typical SELEX methods which are limited to one or a few protein targets.
- *Abstract
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The technology utilizes micro-column affinity chromatography, which requires very little chromatography resin material and is compatible with standard lab equipment and sample processing techniques.
Selection of new RNA aptamers for multiple protein targets was successfully demonstrated using the new micro-column device, resulting in a selected bulk library with significantly better binding affinities based on gel-shift assays. Individual aptamers for each target were identified via high-throughput sequencing analysis of the selected libraries. The technology promises to facilitate the development of high throughput and efficient aptamer selection procedures.
Potential Applications
- drug target validation
- pre-packaged kits
- therapeutics
- chemical analysis
- biotechnology
- chemical separations
- environmental
Advantages
- requires very little sample material
- compatible with standard equipment and processing (syringe pumps, 96-well plates, etc.)
- fabricated from inexpensive, reusable materials
- optically transparent, allowing for monitoring process steps
- configurable to accommodate different aptamer selections
- *Licensing
- Martin Teschlmt439@cornell.edu(607) 254-4454
- 其他
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- Patent application: WO/2013/170256
- Latulippe, D. R.; Szeto, K.; Ozer,A.; Duarte, F. M.; Kelly, C. V.; Pagano, J. M.; White, B. S.; Shalloway, D.;Lis, J. T.; Craighead, H. G., “Multiplexedmicrocolumn-based process for efficient selection of RNA aptamers," Analytical Chemistry 2013
- Latulippe, D. R.; Szeto, K.; Ozer, A.; Pagano, J.;Kelly, C., Lis, J.T.; Craighead, H.G., “Advancedcharacterization of a novel multitarget microscale fluidic device for theselection of RNA aptamers,” 244th ACSNational Meeting & Exposition, Pub. 188 (2012)
- 國家/地區
- 美國
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