Polyclonal Antibodies to Tctex-1
- *Abstract
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Immune rabbit serum against bovine tctex-1 for purification of polyclonal antibodies (pAbs) (1). Bovine tctex-1 shares 100% amino acid sequence identity with its human homolog. pAbs cross-react with tctex-1 of mouse, rat, and human origin. Tctex-1 is the light subunit of dynein, involved in cargo-binding in neurons. It was recently shown to be a marker of neural progenitor cells (2).
These pAbs have been used as neuroscience reagents to study neurogenesis (3), and cytoskeletal remodeling (4) and dynamics (5,6). However, due to the wide organ expression of Tctex-1 (EST profile is here) (7) anti-Tctex-1 pAbs are also useful reagents outside of the field of neuroscience (8). Anti-tctex-1 antibodies applications: Western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence.
Modified excerpt from (1) Tctex-1 localization in interphase NRK fibroblasts visualized by immunofluorescent staining NRK cells were briefly extracted with 0.2% Triton X-100 prior to 2% PFA fixation. A and B, NRK fibroblasts were double-labeled with anti-Tctex-1 (A) or anti-a-tubulin (B) mAb. Tctex-1 was observed at the nucleus and at juxtanuclear tubulo-vesicular, Golgi-like structures. Punctate anti-Tctex-1 staining also decorated microtubules. C and D, shown is a single NRK fibroblast stained with anti-Tctex-1 antibody and photographed in two different focal planes, illustrating regular punctate staining in the area of the nuclear envelope. Bars=10 m.
Modified figure from (9). Specific detection of Tctex-1 and RP3 in rat brain lysate, transfected cells, and immunoprecipitates and assembly of Tctex-1 and RP3 in distinct dynein complexes. A, Rat brain lysate was resolved on SDS-PAGE and immunoblotted with anti-Tctex-1 or anti-RP3 antibody. Both antibodies recognized a single ~14 kDa molecule, although Tctex-1 migrated slightly faster than RP3 on SDS-PAGE. C, Cytoplasmic dynein complexes were immunoprecipitated from brain lysates with anti-Tctex-1 or anti-RP3 antibody. The immunoprecipitates were separated on SDS-PAGE and transferred to nitrocellulose. The top half of the filter was immunoblotted with anti-dynein intermediate chain (DIC) to demonstrate that approximately equal amounts of the dynein complex were precipitated by each antibody (top panel). The bottom half of the nitrocellulose filter was immunoblotted with anti-Tctex-1 or anti-RP3 antibody. Anti-Tctex-1 antibody detected Tctex-1 in the anti-Tctex-1 precipitate, but not in the anti-RP3 precipitate. Likewise, anti-RP3 detected RP3 in the anti-RP3 precipitate, but not in the anti-Tctex-1 precipitate. This result suggests that the dynein complex precipitated by Tctex-1 antibody did not contain RP3, and vice versa. Furthermore, Tctex-1 antibody and RP3 antibody did not cross-react with the antigens of each other.
If you are with a reagent company and would be interested in adding this to your product line, please contact Dan-Oscar Antson as per the contact information below.
- *Licensing
- Dan-Oscar Antsonda429@cornell.edu212-746-1297
- 其他
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- Tai AW, et al (1998) Localization of Tctex-1, a cytoplasmic dynein light chain, to the Golgi apparatus and evidence for dynein complex heterogeneity. J Biol Chem. 273(31):19639-49.
- Dedesma, C., et al. (2006) J Comp Neurol 496, 773-786
- Gauthier-Fisher, A.et al. (2009) Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells. Nat Neurosci 12, 735-744
- Chuang JZ et al (2005) The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth. Dev Cell. 9(1):75-86.
- Vlach, J., et al. (2008) D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein.Proc Natl Acad Sci U S A 105, 10565-10570
- Lai, M., et al. (2005) A tctex1-Ca2+ channel complex for selective surface expression of Ca2+ channels in neurons. Nat Neurosci 8, 435-442
- King, S. M., et al. (1996) The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein.. J Biol Chem 271, 32281-32287
- Machado, R. D., et al. (2003) Functional interaction between BMPR-II and Tctex-1, a light chain of Dynein, is isoform-specific and disrupted by mutations underlying primary pulmonary hypertension.Hum Mol Genet 12, 3277-3286
- Chuang JZ, Milner TA, Sung CH. (2001) Subunit heterogeneity of cytoplasmic dynein: Differential expression of 14 kDa dynein light chains in rat hippocampus. J Neurosci. 21(15):5501-12.
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- 美國
