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Non-thermal Cycling for Polymerase Chain Reaction (PCR)

詳細技術說明

University researchers have developed a PCR method and device that does not rely on thermal cycling. The invention can be implemented on a fluidic chip platform and is compatible with standard sample preparation and detection schemes; it offers a totally integrated approach that enables overall reaction efficiency, reduced power consumption and device portability.

*Abstract

PCR is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to facilitate DNA melting and enzymatic replication (switching between double and single stranded DNA). Heating/cooling limits device design and thermocycling is a power-hungry process so that isothermal approaches have been sought as improvements to conventional PCR.

*IP Issue Date

Aug 9, 2016

*Principal Investigation

Name: Sadik Esener

Department:

Name: Inanc Ortac

Department:

附加資料

Inventor: HUANG, Jiandong | TANNER, Julian, Alexander | ZHANG, Xinmei
Priority Number: WO2005010179A1
IPC Current: C12N001510
Assignee Applicant: The University of Hong Kong
Title: METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES | PROCEDE DE CONSTRUCTION ET DE MODIFICATION DE GRANDES MOLECULES D'ADN
Usefulness: METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES | PROCEDE DE CONSTRUCTION ET DE MODIFICATION DE GRANDES MOLECULES D'ADN
Summary: The method is useful for bacterial artificial chromosome manipulation to aid in future studies of gene function in the post-genomic era. The method is useful for engineering any large DNA fragments or inserting large DNA fragments into the Escherichia coli chromosome. It is also useful for constructing and modifying much larger DNA molecules such as human artificial chromosomes.
Novelty: Combining overlapping DNA molecules, useful for human artificial chromosome manipulation for gene function study, by inserting a first DNA fragment from a DNA-vector complex into a second DNA fragment by homologous recombination

主要類別

生物醫學

細分類別

DNA /基因工程

申請號碼

9410171

其他

Intellectual Property Info

The invention is available for licensing and research sponsorship.

Tech ID/UC Case

23227/2011-366-0

Related Cases

2011-366-0

國家/地區

美國